ABSTRACT
Objective To investigate the status of iodine nutrition and variations of thyroid hormone levels in pregnant women in Foshan and to provide the science theory on the clinical diet supplement rational .Methods The samples were collected from 442 ca‐ses of pregnant women in Chancheng district center hospital from June 2013 to November 2014 .Free triiodothyronine(FT3) ,free thyroxine(FT4) and thyroid stimulating hormone(TSH) were determined by method of electrochemiluminescence .The urinary io‐dine content in pregnant women was measured by using cold digestion method according to iodine catalytic effect of Arsenic‐Ceri‐um .Results The median urinary iodine in 442 cases of pregnant women was 174μg/L .The percentage of midrange iodine deficien‐cy ,mild iodine deficiency ,iodine sufficiency and iodine overdose in 442 cases of pregnant women were respectively 4 .30% ,29 .86% , 39 .59% and 26 .24% .First trimester ,second trimester ,third trimester with abnormal level of urine iodine was respectively 72 .41% ,45 .89% ,62 .91% .The different gestational age with abnormal level of urine iodine and normal level of urine iodine was significant differences(P<0 .05) .The urine iodine sufficient group of FT3 ,TSH were lower than the urine iodine deficiency groups and the urine iodine excess group ,The FT4 of urine iodine sufficient group compare with iodine deficiency groups was significant differences(P<0 .05) .TSH in urine iodine sufficient group to compared urine iodine excess group ,with significant differences(P<0 .05) .Conclusion The abnormal rate of the level of iodine nutrition and the prevalence of thyroid diseases of pregnant women in FoShan are relatively high .The pregnant women should be screening and intervention in time to raise healthier .
ABSTRACT
Objective:To construct an eukaryotic expressing plasmid of IL-24,and investigate its inhibitory effects on the growth of tumor cells in vitro and in vivo.Methods:The eukaryotic expressing plasmid of IL-24(pEGFP-C1-IL-24) was constructed by DNA recombination technique.The recombination plasmid and empty vector were transfected into HIC cells,respectively with Lipofectamine 2000 Reagent and the expressions were determined by LSCM;the proliferation of HIC cells was measured by MTT assay;and apoptosis rate and cell-cycle distribution of HIC cells were measured with Flow Cytometry.Mice were inoculated with B16 cells,which were transfected with pEGFP-C1-IL-24 or empty vector,and the tumor size in mice was detected.The inhibitory effect of IL-24 gene transfection in mice solid tumor was observed and measured.Results:The expression of pEGFP-C1-IL-24 in HIC cells was determined by LSCM after the transfection of pEGFP-C1-IL-24.Comparing to the control group,the proliferation of HIC cells was inhibited by transfection with pEGFP-C1-IL-24 and the G2-M phase of the transfected cells was also increased.Moreover the percentage of mice with detectable tumor was significantly decreased after inoculated with B16 cells transfected with pEGFP-C1-IL-24.Growth rate of tumor in mice model was also significantly inhibited in IL-24 gene therapy group as compared with the control grouop(P